Five Ohio teenage boys have been charged by police for playing a cruel trick on a 15-year-old autistic boy.

The suspects, who are all between 14 and 16 years old, allegedly dumped a bucket filled with urine, tobacco spit and water on their autistic classmate in an "ice bucket challenge" prank. They then posted the malicious prank on Instagram, authorities said.

On Tuesday, the five teens appeared in juvenile court and three were charged with two counts of delinquency, assault and disorderly conduct. The other two were each charged with a single count of disorderly conduct, according to the Columbus Dispatch.

"The victim and the five charged juveniles were and are friends and classmates" who attend Bay High School," said prosecutor Duane Deskins in a statement, reports Cleveland.com. "They regularly associate with one another and, at times, engage in distasteful and sophomoric pranks. However, this incident is clearly different. It crossed a moral and legal line, and even the five alleged perpetrators understand that and have expressed regret," he added.

The incident took place on Aug. 18 in Bay Village in Cleveland. Weeks later, the victim's mother found a video of it on her son's cellphone and reported it to police on Sept. 3, she told CNN affiliate WJW-TV.

In the video, the victim is standing outside in his underwear as someone from the rooftop dumps a bucket of murky brown fluid on him. The stunt appears to be a spoof of the ALS ice bucket challenge where participants get a bucket of ice water dumped over their heads in effort to raise awareness and funding for Amyotrophic lateral sclerosis.

"He was embarrassed because he did not know what the contents were until afterwards, and then he didn't want anybody to know," said the mother about her son. "They used his phone to tape it, and they put it up on Instagram."

The mother posted the video on YouTube and encouraged media outlets and viewers to share it in order to catch the perpetrators.